Our journey led us through narrowing tunnels of greenery until we stopped, donned galoshes and squished off the road into the thicket to take samples. Our field site is adjacent to a small, swampy lagoon of crude oil. We came to take soil samples for laboratory analysis to follow the progress of our site. Fire ants plagued our colleague, Ricardo, as we checked the small mountain of sawdust-filled burlap bags for mycelial growth.
The mushrooms died. It happens. As far as we can tell, the mycelium drowned. However, the last time samples were taken, there was a large shade cloth above the site and some happy oysters fruiting. The shade cloth was nowhere to be found and was likely stolen by someone who thought of a more immediate use for it besides sheltering some oil-munching mushrooms. Luckily, this is science. There are no failures, only lessons to be applied to the next experiment. Who could have predicted that water would be a problem in a rainforest?
We drove to another location where our new friend Donald had kindly allowed our team to transform his chicken coop into an ex situ experimental mushrooms site. Donald is an interesting man for another blog entry. Unfortunately, even in a chicken coop, those mushrooms drowned as well. They were being watered, but mushroom cultivation under any circumstances is somewhat of an art. A little care here, a little neglect there. Too much care, in the form of water, seemed to be the culprit here as well. Evolutionarily speaking, fungi are closer related to animals than they are to plants. This is reflected in their basic needs for metabolism: oxygen.
Undeterred, we gathered our samples and took the eight-hour bus ride back to Quito. That’s right, a short flight saves one the narrow roads and switchbacks that climb the 9,000 feet back up to Quito. However, the breathtaking country view and the chance to see Sharon Stone classic “The Quick and the Dead” in Spanish almost makes up for the butt-ache caused by such travel.
Later in the week, we had the unique opportunity of going out into a mountainous rainforest near a place called Nono to collect mushrooms with two local university students and Paul, Ecuador’s only Ecuadorian mushroom taxonomist. Accompanied by Ricardo, a 30-year veteran mushroom cultivator, we were out mushroom hunting with the two most knowledgeable mycologists in Ecuador. Stopping only to drop $2.50 on a 2 liter Coca Cola bottle filled with high-octane sugarcane liquor, we were well equipped to seek out strange new life forms.
Mycologist David Hawksworth has described the staggering biodiversity of the fungal kingdom by explaining that while there are 2,000 species of flowering plants in Great Britain, there are about 12,000 known species of fungi, with species still being characterized in that country. Great Britain happens to be the most well documented places on the planet when it comes to the species living there. While there are somewhere around 250,000 species of flowering plants globally, applying this 6:1 ratio of fungi to plants in Britain, Hawksworth estimates that there are probably 1,500,000 species of fungi worldwide. Of that (admittedly rough) figure, a mere 70,000-100,000 species have been discovered. While I am not an aspiring taxonomist, knowing your mushrooms can be rewarding. To enter the forest where we were doing our investigations, we crossed the bordering cow pastures encountering Ecuadorian ‘Hongos de Vaca’, which are hallucinogenic. Here’s a picture:
Back in Quito, we sat down for the next two days and hammered out a new methodological design to address the issues with our original set-up. It includes some heavy excavation and the expansion of the earlier mentioned chicken-coop to accommodate more ex situ boxes than before. To overcome the water issues that will no doubt continue to harass our efforts to give our oil-supping oysters a nice home, we will be constructing a roof over our new other field site and locating it on private land. We will also be traveling back and forth every two weeks to make sure our fungal friends have everything they need. In short, we will be spending a lot of time in Lago during March. Here’s some pictures of home: